Evidence that histaminergic neurons are devoid of estrogen receptor alpha in the ewe diencephalon during the breeding season.
Gen Comp Endocrinol
; 199: 86-93, 2014 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-24472706
ABSTRACT
In sheep as in rat, it has been highly suggested that neuronal histamine (HA) participates to the estradiol (E2)-induced GnRH and LH surges, through H1 receptor. With the aim of determining if E2 could act directly on HA neurons, we examined here whether HA neurons express estrogen receptor alpha (ERα) in the ewe diencephalon during the breeding season. We first produced a specific polyclonal antibody directed against recombinant ovine histidine decarboxylase (oHDC), the HA synthesizing enzyme. Using both this anti-oHDC antibody and an anti-ERα monoclonal antibody in double label immunohistochemistry, we showed that HA neurons do not express ERα in diencephalon of ewes with different hormonal status. This result diverges from those obtained in rat, in which around three quarters of HA neurons express ERα in their nucleus. This discrepancy between these two mammal species may reflect difference in their neuronal network.
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Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Estaciones del Año
/
Cruzamiento
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Ovinos
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Histamina
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Receptor alfa de Estrógeno
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Diencéfalo
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Gen Comp Endocrinol
Año:
2014
Tipo del documento:
Article