Miro-1 links mitochondria and microtubule Dynein motors to control lymphocyte migration and polarity.
Mol Cell Biol
; 34(8): 1412-26, 2014 Apr.
Article
en En
| MEDLINE
| ID: mdl-24492963
ABSTRACT
The recruitment of leukocytes to sites of inflammation is crucial for a functional immune response. In the present work, we explored the role of mitochondria in lymphocyte adhesion, polarity, and migration. We show that during adhesion to the activated endothelium under physiological flow conditions, lymphocyte mitochondria redistribute to the adhesion zone together with the microtubule-organizing center (MTOC) in an integrin-dependent manner. Mitochondrial redistribution and efficient lymphocyte adhesion to the endothelium require the function of Miro-1, an adaptor molecule that couples mitochondria to microtubules. Our data demonstrate that Miro-1 associates with the dynein complex. Moreover, mitochondria accumulate around the MTOC in response to the chemokine CXCL12/SDF-1α; this redistribution is regulated by Miro-1. CXCL12-dependent cell polarization and migration are reduced in Miro-1-silenced cells, due to impaired myosin II activation at the cell uropod and diminished actin polymerization. These data point to a key role of Miro-1 in the control of lymphocyte adhesion and migration through the regulation of mitochondrial redistribution.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos
/
Polaridad Celular
/
Dineínas
/
Proteínas de Unión al GTP rho
/
Proteínas Mitocondriales
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Quimiocina CXCL12
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Microtúbulos
/
Mitocondrias
Límite:
Humans
Idioma:
En
Revista:
Mol Cell Biol
Año:
2014
Tipo del documento:
Article
País de afiliación:
España