D-chiro-inositol glycan stimulates insulin secretion in pancreatic ß cells.
Mol Cell Endocrinol
; 387(1-2): 1-7, 2014 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-24530497
ABSTRACT
Insulin has been shown to act on pancreatic ß cells to regulate its own secretion. Currently the mechanism underlying this effect is unclear. INS-2, a novel inositol glycan pseudo-disaccharide containing D-chiro-inositol and galactosamine, has been shown to function as an insulin mimetic and a putative insulin mediator. In the present study we found that INS-2 stimulates insulin secretion in MIN6 ß cells and potentiates glucose stimulated insulin secretion in isolated mouse islets. Importantly, INS-2 failed to potentiate insulin secretion induced by tolbutamide, which stimulates insulin release by closing ATP sensitive potassium channels (KATP). Electrophysiological studies showed that INS-2 inhibited sulfonylurea-sensitive KATP conductance. The effect of INS-2 on inhibiting KATP channel is mediated by protein phosphatase 2C (PP2C), as knocking down PP2C expression in MIN6 cells by PP2C small hairpin RNA completely abolished the effect of INS-2 on KATP and consequently attenuated INS-2 induced insulin secretion. In conclusion, the present study identifies a novel mechanism involving PP2C in regulating KATP channel activity and consequently insulin secretion.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfoproteínas Fosfatasas
/
Células Secretoras de Insulina
/
Canales KATP
/
Insulina
Límite:
Animals
Idioma:
En
Revista:
Mol Cell Endocrinol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos