Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment.
Virology
; 452-453: 202-11, 2014 Mar.
Article
en En
| MEDLINE
| ID: mdl-24606697
ABSTRACT
We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Virus de la Fiebre Amarilla
/
Productos del Gen gag
/
Infecciones por VIH
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Virus de la Inmunodeficiencia de los Simios
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Vacunas contra el SIDA
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Virology
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos