Rapid purification of gram quantities of ß-sitosterol from a commercial phytosterol mixture.
BMC Res Notes
; 7: 182, 2014 Mar 27.
Article
en En
| MEDLINE
| ID: mdl-24674386
ABSTRACT
BACKGROUND:
ß-Sitosterol, a plant sterol or phytosterol, has commercial uses in the nutraceutical and pharmaceutical industries, but is also employed frequently in biological research. Phytosterols always accumulate as mixtures, and obtaining highly pure ß-sitosterol in larger quantities for biological assays has been a challenge.FINDINGS:
An improved method for the rapid purification of ß-sitosterol from a commercial phytosterol extract is presented. Fractional crystallization of soybean oil yielded a soluble and an insoluble fraction. ß-Sitosterol was purified by silica gel and Na-Y zeolite chromatography.CONCLUSION:
The rapid and cost effective three-step purification described here afforded ß-sitosterol in gram quantities with high purity (>92%) and yield (>22%).
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sitoesteroles
/
Aceite de Soja
Idioma:
En
Revista:
BMC Res Notes
Año:
2014
Tipo del documento:
Article