Scalable production of highly sensitive nanosensors based on graphene functionalized with a designed G protein-coupled receptor.
Nano Lett
; 14(5): 2709-14, 2014 May 14.
Article
en En
| MEDLINE
| ID: mdl-24742304
ABSTRACT
We have developed a novel, all-electronic biosensor for opioids that consists of an engineered µ-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of â¼1500 cm(2) V(-1) s(-1) and yield exceeding 98%. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Receptores Opioides mu
/
Grafito
/
Naltrexona
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Nano Lett
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos