Inhibitory effects of the platelet-activating factor receptor antagonists, CV-3988 and Ginkgolide B, on alkali burn-induced corneal neovascularization.
Cutan Ocul Toxicol
; 34(1): 53-60, 2015 Mar.
Article
en En
| MEDLINE
| ID: mdl-24754407
ABSTRACT
PURPOSE:
Platelet-activating factor (PAF) has been found in various ocular tissues; the activity of PAF depends on the binding to its specific receptor, PAF-receptor. We investigated the therapeutic effects of PAF-receptor antagonists (CV-3988 and Ginkgolide B) on alkali burn-induced corneal neovascularization (CNV).METHODS:
CNV was induced by applying a 0.2 N sodium hydroxide (3 µl, NaOH) solution directly on mice corneas. CV-3988 (1 mM/10 µl) and Ginkgolide B (1 mM/10 µl) were administered topically on the corneas three times daily for three consecutive days. CNV was evaluated under a slit-lamp microscope. Corneas were processed for histological, immunohistochemical and reverse transcription polymerase chain reaction analysis. Human umbilical vein endothelial cells were used for the migration and tube formation assay.RESULTS:
Application of CV-3988 and Ginkgolide B inhibited CNV caused by alkali burn. CV-3988 and Ginkgolide B attenuated the expression of PAF-receptor mRNA. Alkali injury induced a massively increased intraocular mRNA expression of an angiogenic factor in cornea tissues, whereas these increments were attenuated by the application of CV-3988 and Ginkgolide B.CONCLUSIONS:
CV-3988 and Ginkgolide B reversed opacity and neovascularization in alkali burn-induced corneas. Our findings suggest that CV-3988 and Ginkgolide B may be therapeutically useful in the treatment of CNV and inflammation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Éteres Fosfolípidos
/
Inhibidores de Agregación Plaquetaria
/
Glicoproteínas de Membrana Plaquetaria
/
Quemaduras Oculares
/
Neovascularización de la Córnea
/
Receptores Acoplados a Proteínas G
/
Ginkgólidos
/
Lactonas
Límite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Cutan Ocul Toxicol
Asunto de la revista:
DERMATOLOGIA
/
OFTALMOLOGIA
/
TOXICOLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos