UM-SCC-103: a unique tongue cancer cell line that recapitulates the tumorigenic stem cell population of the primary tumor.
Ann Otol Rhinol Laryngol
; 123(9): 662-72, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-24816422
ABSTRACT
OBJECTIVE:
A new head and neck cancer cell line was developed from a highly aggressive HNSCC of the oral cavity diagnosed in a 26-year-old pregnant woman.METHODS:
Cells from the primary tumor were passaged in culture and genotyped as a unique cell line. The resultant cell line was assessed for its ability to replicate the primary tumor.RESULTS:
The primary tumor and cell line contained 19.03% and 19.62% CD44(high) cells, respectively. CD44(high) cancer stem cells from UM-SCC-103 formed tumors after flank injections in mice that reconstituted the heterogeneity of the primary tumor. CD44 staining and histology in the primary tumor and tumors grown in vivo from the cell line were similar. CD44(high) cells from the primary tumor resulted in lung colony formation in 2 out of 2 tail vein injections in mice, whereas CD44(low) cells did not. Similarly, CD44(high) cells from UM-SCC-103 formed lung tumors in 2 out of 4 mice, whereas CD44(low) cells did not.CONCLUSION:
The similarity in marker expression and tumorigenic behavior between the primary tumor and the resulting cell line strongly suggests that the cell line resembles the primary tumor that it was derived from and provides an important new research tool for the study of head and neck carcinomas in young patients.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Lengua
/
Carcinoma de Células Escamosas
/
Biomarcadores de Tumor
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Regulación Neoplásica de la Expresión Génica
/
Receptores de Hialuranos
Límite:
Adult
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Animals
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Ann Otol Rhinol Laryngol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos