A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1.
BMC Biotechnol
; 14: 51, 2014 May 30.
Article
en En
| MEDLINE
| ID: mdl-24885867
ABSTRACT
BACKGROUND:
Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp.RESULTS:
We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1.CONCLUSION:
pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Streptomyces
/
Bacteriófagos
/
Vectores Genéticos
País/Región como asunto:
America do sul
/
Venezuela
Idioma:
En
Revista:
BMC Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2014
Tipo del documento:
Article