Sensitivity of real-time PCR performed on dried sera spotted on filter paper for diagnosis of leptospirosis.
J Clin Microbiol
; 52(8): 3075-7, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-24899024
ABSTRACT
In remote countries, leptospirosis confirmation is difficult because it requires the shipment of frozen samples to reference laboratories. The sensitivity of leptospirosis real-time PCR performed on filter paper-dried serum samples stored at ambient temperature was evaluated at 2 × 10(2) equivalent leptospires/ml (eqLeptospires/ml). This easy alternative procedure can be used to enhance the surveillance of leptospirosis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Manejo de Especímenes
/
Técnicas de Diagnóstico Molecular
/
Desecación
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Leptospirosis
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Año:
2014
Tipo del documento:
Article