The Mn4Ca photosynthetic water-oxidation catalyst studied by simultaneous X-ray spectroscopy and crystallography using an X-ray free-electron laser.
Philos Trans R Soc Lond B Biol Sci
; 369(1647): 20130324, 2014 Jul 17.
Article
en En
| MEDLINE
| ID: mdl-24914152
ABSTRACT
The structure of photosystem II and the catalytic intermediate states of the Mn4CaO5 cluster involved in water oxidation have been studied intensively over the past several years. An understanding of the sequential chemistry of light absorption and the mechanism of water oxidation, however, requires a new approach beyond the conventional steady-state crystallography and X-ray spectroscopy at cryogenic temperatures. In this report, we present the preliminary progress using an X-ray free-electron laser to determine simultaneously the light-induced protein dynamics via crystallography and the local chemistry that occurs at the catalytic centre using X-ray spectroscopy under functional conditions at room temperature.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría por Rayos X
/
Difracción de Rayos X
/
Modelos Moleculares
/
Compuestos de Manganeso
/
Compuestos de Calcio
/
Complejo de Proteína del Fotosistema II
/
Luz
Idioma:
En
Revista:
Philos Trans R Soc Lond B Biol Sci
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos