Bacterial nanoscale cultures for phenotypic multiplexed antibiotic susceptibility testing.
J Clin Microbiol
; 52(9): 3310-7, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-24989602
ABSTRACT
An optimal antimicrobial drug regimen is the key to successful clinical outcomes of bacterial infections. To direct the choice of antibiotic, access to fast and precise antibiotic susceptibility profiling of the infecting bacteria is critical. We have developed a high-throughput nanowell antibiotic susceptibility testing (AST) device for direct, multiplexed analysis. By processing in real time the optical recordings of nanoscale cultures of reference and clinical uropathogenic Escherichia coli strains with a mathematical algorithm, the time point when growth shifts from lag phase to early logarithmic phase (Tlag) was identified for each of the several hundreds of cultures tested. Based on Tlag, the MIC could be defined within 4 h. Heatmap presentation of data from this high-throughput analysis allowed multiple resistance patterns to be differentiated at a glance. With a possibility to enhance multiplexing capacity, this device serves as a high-throughput diagnostic tool that rapidly aids clinicians in prescribing the optimal antibiotic therapy.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pruebas de Sensibilidad Microbiana
/
Escherichia coli Uropatógena
/
Ensayos Analíticos de Alto Rendimiento
/
Antibacterianos
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Suecia