Potato leaf extract and its component, α-solanine, exert similar impacts on development and oxidative stress in Galleria mellonella L.
Arch Insect Biochem Physiol
; 87(1): 26-39, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-25041927
ABSTRACT
Plants synthesize a broad range of secondary metabolites that act as natural defenses against plant pathogens and herbivores. Among these, potato plants produce glycoalkaloids (GAs). In this study, we analyzed the effects of the dried extract of fresh potato leaves (EPL) on the biological parameters of the lepidopteran, Galleria mellonella (L.) and compared its activity to one of the main EPL components, the GA α-solanine. Wax moth larvae were reared from first instar on a diet supplemented with three concentrations of EPL or α-solanine. Both EPL and α-solanine affected survivorship, fecundity, and fertility of G. mellonella to approximately the same extent. We evaluated the effect of EPL and α-solanine on oxidative stress in midgut and fat body by measuring malondialdehyde (MDA) and protein carbonyl (PCO) contents, both biomarkers of oxidative damage. We evaluated glutathione S-transferase (GST) activity, a detoxifying enzyme acting in prevention of oxidative damage. EPL and α-solanine altered MDA and PCO concentrations and GST activity in fat body and midgut. We infer that the influence of EPL on G. mellonella is not enhanced by synergistic effects of the totality of potato leaf components compared to α-solanine alone.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Solanina
/
Solanum tuberosum
/
Extractos Vegetales
/
Estrés Oxidativo
/
Tracto Gastrointestinal
/
Fertilidad
/
Larva
/
Mariposas Nocturnas
Límite:
Animals
Idioma:
En
Revista:
Arch Insect Biochem Physiol
Asunto de la revista:
BIOLOGIA
/
BIOQUIMICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Polonia