Strategy to identify and quantify polysaccharide gums in gelled food concentrates.
Food Chem
; 166: 42-49, 2015 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-25053026
ABSTRACT
A strategy for the unambiguous identification and selective quantification of xanthan gum and locust bean gum (LBG) in gelled food concentrates is presented. DNA detection by polymerase chain reaction (PCR) showed to be a fast, sensitive, and selective method that can be used as a first screening tool in intact gelled food concentrates. An efficient isolation procedure is described removing components that may interfere with subsequent analyses. NMR spectroscopy enabled the direct identification of xanthan gum and the discrimination between different galactomannans in the isolated polysaccharide fraction. An enzymatic fingerprinting method using endo-ß-mannanase, in addition to being used to differentiate between galactomannans, was developed into a selective, quantitative method for LBG, whereas monosaccharide analysis was used to quantify xanthan gum. Recoveries for xanthan gum and LBG were 87% and 70%, respectively, with in-between day relative standard deviations below 20% for xanthan gum and below 10% for LBG.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polisacáridos
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Polisacáridos Bacterianos
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Espectroscopía de Resonancia Magnética
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Gomas de Plantas
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Galactanos
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Mananos
Idioma:
En
Revista:
Food Chem
Año:
2015
Tipo del documento:
Article