Analyzing gene function in adult long-term hematopoietic stem cells using the interferon inducible Mx1-Cre mouse system.
Methods Mol Biol
; 1194: 313-25, 2014.
Article
en En
| MEDLINE
| ID: mdl-25064111
Long-term hematopoietic stem cells (LT-HSCs) have the ability to self-renew and differentiate into all blood cell lineages. Understanding the genetic networks that regulate LT-HSC function in the adult bone marrow requires inducible gene targeting and bone marrow transplantations. In this chapter we describe the use of the inducible Mx1-Cre mouse model to delete genes in LT-HSCs and methodologies for examining the function of LT-HSCs following deletion.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Madre Hematopoyéticas
/
Interferones
/
Marcación de Gen
/
Integrasas
/
Células Madre Adultas
/
Proteínas de Resistencia a Mixovirus
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2014
Tipo del documento:
Article