Decoration of silicon nanostructures with copper particles for simultaneous selective capture and mass spectrometry detection of His-tagged model peptide.
Analyst
; 139(20): 5155-63, 2014 Oct 21.
Article
en En
| MEDLINE
| ID: mdl-25112364
ABSTRACT
We present in this work a simple and fast preparation method of a new affinity surface-assisted laser/desorption ionization mass spectrometry (SALDI-MS) substrate based on silicon nanostructures decorated with copper particles. The silicon nanostructures were fabricated by the metal-assisted chemical etching (MACE) method. Then, superhydrophilic areas surrounded by superhydrophobic regions were formed through hydrosilylation reaction of 1-octadecene, followed by local degradation of the octadecyl layer. After that, copper particles were deposited in the hydrophilic areas by using the electroless method. We have demonstrated that these surfaces were able to perform high selective capture of model His-tag peptide even in a complex mixture such as serum solution. Then, the captured peptide was detected by mass spectrometry at a femtomolar level without the need of organic matrix.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
/
Silicio
/
Técnicas de Química Analítica
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Cobre
/
Nanoestructuras
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Analyst
Año:
2014
Tipo del documento:
Article
País de afiliación:
Francia