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C19MC microRNAs regulate the migration of human trophoblasts.
Xie, Lan; Mouillet, Jean-Francois; Chu, Tianjiao; Parks, W Tony; Sadovsky, Elena; Knöfler, Martin; Sadovsky, Yoel.
Afiliación
  • Xie L; Magee-Womens Research Institute (L.X., J.-F.M., T.C., W.T.P., E.S., Y.S.), Department of OBGYN and Reproductive Sciences, University of Pittsburgh, Pennsylvania 15213; Medical Systems Biology Research Center (L.X.), Department of Biomedical Engineering, Tsinghua University School of Medicine, Beijing 100084 China; Department of Obstetrics and Fetal-Maternal Medicine (M.K.), Reproductive Biology Unit, Medical University of Vienna, Vienna, A-1090 Austria; and Department of Microbiology and Molecul
Endocrinology ; 155(12): 4975-85, 2014 Dec.
Article en En | MEDLINE | ID: mdl-25211593
Early in pregnancy, trophoblast invasion into the decidua and inner myometrium is essential for establishment of proper implantation, maternal-fetal exchange, and immunological tolerance of the feto-placental allograft. Unlike villous trophoblasts (VTs), extravillous trophoblasts (EVTs) are unique in their capacity to invade the maternal decidua and myometrium. The largest human microRNA (miRNA) gene cluster, the chromosome 19 miRNA cluster (C19MC), is expressed almost exclusively in the placenta and, rarely, in certain tumors and undifferentiated cells. In the work reported here, we found that the expression of C19MC miRNAs is higher in VTs than in EVTs. Using a bacterial artificial chromosome (BAC)-mediated overexpression of C19MC miRNAs in an EVT-derived cell line, which does not naturally express these miRNAs, we found that C19MC miRNAs selectively attenuate cell migration without affecting cell proliferation or apoptosis. A microarray analysis revealed that C19MC miRNAs regulate target transcripts related to cellular movement. Our data also implicated a specific C19MC member, miR-519d, indirectly regulating the EVT invasive phenotype by targeting CXCL6, NR4A2 and FOXL2 transcripts through a 3'UTR miRNA-responsive element. Together, our data suggest a role for C19MC miRNAs in modulating the migration of EVTs.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Trofoblastos / Cromosomas Humanos Par 19 / MicroARNs Límite: Female / Humans / Pregnancy Idioma: En Revista: Endocrinology Año: 2014 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Trofoblastos / Cromosomas Humanos Par 19 / MicroARNs Límite: Female / Humans / Pregnancy Idioma: En Revista: Endocrinology Año: 2014 Tipo del documento: Article