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An optical clearing technique for plant tissues allowing deep imaging and compatible with fluorescence microscopy.
Warner, Cherish A; Biedrzycki, Meredith L; Jacobs, Samuel S; Wisser, Randall J; Caplan, Jeffrey L; Sherrier, D Janine.
Afiliación
  • Warner CA; Delaware Biotechnology Institute (C.A.W., S.S.J., J.C., D.J.S.) andDepartments of Biological Sciences (C.A.W., M.L.B., S.S.J., J.C., D.J.S.); andPlant and Soil Sciences (M.L.B., R.J.W., J.C., D.J.S.), University of Delaware, Newark, Delaware 19717-1303 jcaplan@udel.edu sherrier@dbi.udel.edu.
  • Biedrzycki ML; Delaware Biotechnology Institute (C.A.W., S.S.J., J.C., D.J.S.) andDepartments of Biological Sciences (C.A.W., M.L.B., S.S.J., J.C., D.J.S.); andPlant and Soil Sciences (M.L.B., R.J.W., J.C., D.J.S.), University of Delaware, Newark, Delaware 19717-1303.
  • Jacobs SS; Delaware Biotechnology Institute (C.A.W., S.S.J., J.C., D.J.S.) andDepartments of Biological Sciences (C.A.W., M.L.B., S.S.J., J.C., D.J.S.); andPlant and Soil Sciences (M.L.B., R.J.W., J.C., D.J.S.), University of Delaware, Newark, Delaware 19717-1303.
  • Wisser RJ; Delaware Biotechnology Institute (C.A.W., S.S.J., J.C., D.J.S.) andDepartments of Biological Sciences (C.A.W., M.L.B., S.S.J., J.C., D.J.S.); andPlant and Soil Sciences (M.L.B., R.J.W., J.C., D.J.S.), University of Delaware, Newark, Delaware 19717-1303.
  • Caplan JL; Delaware Biotechnology Institute (C.A.W., S.S.J., J.C., D.J.S.) andDepartments of Biological Sciences (C.A.W., M.L.B., S.S.J., J.C., D.J.S.); andPlant and Soil Sciences (M.L.B., R.J.W., J.C., D.J.S.), University of Delaware, Newark, Delaware 19717-1303 jcaplan@udel.edu sherrier@dbi.udel.edu.
  • Sherrier DJ; Delaware Biotechnology Institute (C.A.W., S.S.J., J.C., D.J.S.) andDepartments of Biological Sciences (C.A.W., M.L.B., S.S.J., J.C., D.J.S.); andPlant and Soil Sciences (M.L.B., R.J.W., J.C., D.J.S.), University of Delaware, Newark, Delaware 19717-1303 jcaplan@udel.edu sherrier@dbi.udel.edu.
Plant Physiol ; 166(4): 1684-7, 2014 Dec.
Article en En | MEDLINE | ID: mdl-25344504
ABSTRACT
We report on a nondestructive clearing technique that enhances transmission of light through specimens from diverse plant species, opening unique opportunities for microscope-enabled plant research. After clearing, plant organs and thick tissue sections are amenable to deep imaging. The clearing method is compatible with immunocytochemistry techniques and can be used in concert with common fluorescent probes, including widely adopted protein tags such as GFP, which has fluorescence that is preserved during the clearing process.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Nicotiana / Pisum sativum / Zea mays / Imagenología Tridimensional / Medicago truncatula Idioma: En Revista: Plant Physiol Año: 2014 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Nicotiana / Pisum sativum / Zea mays / Imagenología Tridimensional / Medicago truncatula Idioma: En Revista: Plant Physiol Año: 2014 Tipo del documento: Article