An optical clearing technique for plant tissues allowing deep imaging and compatible with fluorescence microscopy.
Plant Physiol
; 166(4): 1684-7, 2014 Dec.
Article
en En
| MEDLINE
| ID: mdl-25344504
ABSTRACT
We report on a nondestructive clearing technique that enhances transmission of light through specimens from diverse plant species, opening unique opportunities for microscope-enabled plant research. After clearing, plant organs and thick tissue sections are amenable to deep imaging. The clearing method is compatible with immunocytochemistry techniques and can be used in concert with common fluorescent probes, including widely adopted protein tags such as GFP, which has fluorescence that is preserved during the clearing process.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Nicotiana
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Pisum sativum
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Zea mays
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Imagenología Tridimensional
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Medicago truncatula
Idioma:
En
Revista:
Plant Physiol
Año:
2014
Tipo del documento:
Article