Directed evolution of an ultrastable carbonic anhydrase for highly efficient carbon capture from flue gas.
Proc Natl Acad Sci U S A
; 111(46): 16436-41, 2014 Nov 18.
Article
en En
| MEDLINE
| ID: mdl-25368146
ABSTRACT
Carbonic anhydrase (CA) is one of nature's fastest enzymes and can dramatically improve the economics of carbon capture under demanding environments such as coal-fired power plants. The use of CA to accelerate carbon capture is limited by the enzyme's sensitivity to the harsh process conditions. Using directed evolution, the properties of a ß-class CA from Desulfovibrio vulgaris were dramatically enhanced. Iterative rounds of library design, library generation, and high-throughput screening identified highly stable CA variants that tolerate temperatures of up to 107 °C in the presence of 4.2 M alkaline amine solvent at pH >10.0. This increase in thermostability and alkali tolerance translates to a 4,000,000-fold improvement over the natural enzyme. At pilot scale, the evolved catalyst enhanced the rate of CO2 absorption 25-fold compared with the noncatalyzed reaction.
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01-internacional
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MEDLINE
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En
Revista:
Proc Natl Acad Sci U S A
Año:
2014
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Article