Your browser doesn't support javascript.
loading
Natural genetic variation impacts expression levels of coding, non-coding, and antisense transcripts in fission yeast.
Clément-Ziza, Mathieu; Marsellach, Francesc X; Codlin, Sandra; Papadakis, Manos A; Reinhardt, Susanne; Rodríguez-López, María; Martin, Stuart; Marguerat, Samuel; Schmidt, Alexander; Lee, Eunhye; Workman, Christopher T; Bähler, Jürg; Beyer, Andreas.
Afiliación
  • Clément-Ziza M; Biotechnology Centre, Technische Universität Dresden, Dresden, Germany Cologne Cluster of Excellence in Cellular Stress Responses in Aging-associated Diseases (CECAD), University of Cologne, Cologne, Germany.
  • Marsellach FX; Department of Genetics, Evolution & Environment and UCL Genetics Institute, University College London, London, UK.
  • Codlin S; Department of Genetics, Evolution & Environment and UCL Genetics Institute, University College London, London, UK.
  • Papadakis MA; Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
  • Reinhardt S; Biotechnology Centre, Technische Universität Dresden, Dresden, Germany.
  • Rodríguez-López M; Department of Genetics, Evolution & Environment and UCL Genetics Institute, University College London, London, UK.
  • Martin S; Department of Genetics, Evolution & Environment and UCL Genetics Institute, University College London, London, UK.
  • Marguerat S; Department of Genetics, Evolution & Environment and UCL Genetics Institute, University College London, London, UK.
  • Schmidt A; Biozentrum, University of Basel, Basel, Switzerland.
  • Lee E; Department of Genetics, Evolution & Environment and UCL Genetics Institute, University College London, London, UK.
  • Workman CT; Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
  • Bähler J; Department of Genetics, Evolution & Environment and UCL Genetics Institute, University College London, London, UK j.bahler@ucl.ac.uk andreas.beyer@uni-koeln.de.
  • Beyer A; Biotechnology Centre, Technische Universität Dresden, Dresden, Germany Cologne Cluster of Excellence in Cellular Stress Responses in Aging-associated Diseases (CECAD), University of Cologne, Cologne, Germany j.bahler@ucl.ac.uk andreas.beyer@uni-koeln.de.
Mol Syst Biol ; 10: 764, 2014 Nov 28.
Article en En | MEDLINE | ID: mdl-25432776
Our current understanding of how natural genetic variation affects gene expression beyond well-annotated coding genes is still limited. The use of deep sequencing technologies for the study of expression quantitative trait loci (eQTLs) has the potential to close this gap. Here, we generated the first recombinant strain library for fission yeast and conducted an RNA-seq-based QTL study of the coding, non-coding, and antisense transcriptomes. We show that the frequency of distal effects (trans-eQTLs) greatly exceeds the number of local effects (cis-eQTLs) and that non-coding RNAs are as likely to be affected by eQTLs as protein-coding RNAs. We identified a genetic variation of swc5 that modifies the levels of 871 RNAs, with effects on both sense and antisense transcription, and show that this effect most likely goes through a compromised deposition of the histone variant H2A.Z. The strains, methods, and datasets generated here provide a rich resource for future studies.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Schizosaccharomyces / ARN de Hongos / Proteínas de Ciclo Celular / Proteínas de Schizosaccharomyces pombe Tipo de estudio: Prognostic_studies Idioma: En Revista: Mol Syst Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Schizosaccharomyces / ARN de Hongos / Proteínas de Ciclo Celular / Proteínas de Schizosaccharomyces pombe Tipo de estudio: Prognostic_studies Idioma: En Revista: Mol Syst Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Alemania