Baseline separation of amino acid biomarkers of hepatocellular carcinoma by polyvinylpyrrolidone-filled capillary electrophoresis with light-emitting diode-induced fluorescence in the presence of mixed micelles.
Analyst
; 140(3): 847-53, 2015 Feb 07.
Article
en En
| MEDLINE
| ID: mdl-25460547
ABSTRACT
Physiological amino acids (AAs) are important indices for monitoring various diseases, including cancer. This study proposes a polymer-based separation method in the presence of mixed micelles for the determination of AAs by capillary electrophoresis with light-emitting diode-induced fluorescence. The separation of 18 amino acid-cyano[f]benzoisoindoles (AA-CBIs) was successfully achieved using a solution of polyvinylpyrrolidone (PVP, 5% w/v, Mavg 1,300,000 Da). In addition, we demonstrated that mixed micelles composed of sodium dodecyl sulfate and isopropanol may affect the migration order of the AA-CBIs and greatly improve the speed of separation. With the exception of proline, 21 plasma AA-CBIs, including high isoelectric point AAs (lysine, ornithine, and arginine), were identified by using optimized separation conditions with minimal matrix effects. The results of this study demonstrated the distinct advantages of the proposed method, such as simplicity, high efficiency, and cost-effectiveness. This method has great potential for the diagnosis of several important diseases, including carcinomas, aminoacidopathies, and neurotransmission disorders.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Biomarcadores de Tumor
/
Povidona
/
Carcinoma Hepatocelular
/
Electroforesis Capilar
/
Aminoácidos
/
Luz
/
Neoplasias Hepáticas
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Analyst
Año:
2015
Tipo del documento:
Article
País de afiliación:
Taiwán