[Novel cationic liposome loading siRNA inhibits the expression of hepatitis B virus HBx gene].
Yao Xue Xue Bao
; 49(9): 1326-30, 2014 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-25518334
ABSTRACT
In order to solve the problem of selection and in vivo delivery problem in siRNA treatment, hepatitis B virus (HBV) HBx gene which could be targeted by siRNA was studied. The siRNA expression plasmid which specific inhibits HBx expression was obtained by in vitro selection via a dual-luciferase plasmid including HBx-Fluc fusion protein expression domain. The selected siRNA expression plasmid was then encapsulated in PEG-modified cationic liposome, which was devoted into pharmacodynamic studies at both cellular and animal level. The results illustrated that the cationic liposome which encapsulated siRNA expression plasmid could effectively inhibit HBx gene expression both in vitro and in vivo.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Regulación Viral de la Expresión Génica
/
Transactivadores
/
ARN Interferente Pequeño
/
Liposomas
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Año:
2014
Tipo del documento:
Article