Your browser doesn't support javascript.
loading
A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome.
Chapman, Jarrod A; Mascher, Martin; Buluç, Aydin; Barry, Kerrie; Georganas, Evangelos; Session, Adam; Strnadova, Veronika; Jenkins, Jerry; Sehgal, Sunish; Oliker, Leonid; Schmutz, Jeremy; Yelick, Katherine A; Scholz, Uwe; Waugh, Robbie; Poland, Jesse A; Muehlbauer, Gary J; Stein, Nils; Rokhsar, Daniel S.
Afiliación
  • Chapman JA; Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA. jarrodc@gmail.com.
  • Mascher M; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 06466, Stadt Seeland, Germany. mascher@ipk-gatersleben.de.
  • Buluç A; Computational Research Division and National Energy Research Supercomputing Center (NERSC), Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. abuluc@lbl.gov.
  • Barry K; Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA. kwbarry@lbl.gov.
  • Georganas E; Computational Research Division and National Energy Research Supercomputing Center (NERSC), Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. egeor@eecs.berkeley.edu.
  • Session A; Department of Electrical Engineering and Computer Science, Computer Science Division, University of California, Berkeley, CA, 94720, USA. egeor@eecs.berkeley.edu.
  • Strnadova V; Department of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA. adamsession@berkeley.edu.
  • Jenkins J; Department of Computer Science, University of California, Santa Barbara, CA, 93106, USA. veronika@cs.ucsb.edu.
  • Sehgal S; Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA. jjenkins@hudsonalpha.org.
  • Oliker L; HudsonAlpha Institute of Biotechnology, Huntsville, AL, 35806, USA. jjenkins@hudsonalpha.org.
  • Schmutz J; Department of Plant Pathology, Kansas State University, Manhattan, KS, 65506, USA. sunish.sehgal@sdstate.edu.
  • Yelick KA; Present address: Department of Plant Science, South Dakota State University, Brookings, SD, 57007, USA. sunish.sehgal@sdstate.edu.
  • Scholz U; Computational Research Division and National Energy Research Supercomputing Center (NERSC), Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. loliker@lbl.gov.
  • Waugh R; Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA. jschmutz@hudsonalpha.org.
  • Poland JA; HudsonAlpha Institute of Biotechnology, Huntsville, AL, 35806, USA. jschmutz@hudsonalpha.org.
  • Muehlbauer GJ; Computational Research Division and National Energy Research Supercomputing Center (NERSC), Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. yelick@eecs.berkeley.edu.
  • Stein N; Department of Electrical Engineering and Computer Science, Computer Science Division, University of California, Berkeley, CA, 94720, USA. yelick@eecs.berkeley.edu.
  • Rokhsar DS; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 06466, Stadt Seeland, Germany. scholz@ipk-gatersleben.de.
Genome Biol ; 16: 26, 2015 Jan 31.
Article en En | MEDLINE | ID: mdl-25637298
ABSTRACT
Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population.
Asunto(s)
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Poliploidía / Triticum / Pan / Análisis de Secuencia de ADN / Genoma de Planta Idioma: En Revista: Genome Biol Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Poliploidía / Triticum / Pan / Análisis de Secuencia de ADN / Genoma de Planta Idioma: En Revista: Genome Biol Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos