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Arabidopsis type I proton-pumping pyrophosphatase expresses strongly in phloem, where it is required for pyrophosphate metabolism and photosynthate partitioning.
Pizzio, Gaston A; Paez-Valencia, Julio; Khadilkar, Aswad S; Regmi, Kamesh; Patron-Soberano, Araceli; Zhang, Shangji; Sanchez-Lares, Jonathan; Furstenau, Tara; Li, Jisheng; Sanchez-Gomez, Concepcion; Valencia-Mayoral, Pedro; Yadav, Umesh P; Ayre, Brian G; Gaxiola, Roberto A.
Afiliación
  • Pizzio GA; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Paez-Valencia J; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Khadilkar AS; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Regmi K; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Patron-Soberano A; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Zhang S; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Sanchez-Lares J; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Furstenau T; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Li J; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Sanchez-Gomez C; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Valencia-Mayoral P; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Yadav UP; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Ayre BG; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
  • Gaxiola RA; School of Life Sciences, Arizona State University, Tempe, Arizona 852872 (G.A.P., J.P.-V., K.R., S.Z., J.S.-L., T.F., J.L., R.A.G.);Department of Botany, University of Wisconsin, Madison, Wisconsin 53706 (J.P.-V.);Department of Biological Sciences, University of North Texas, Denton, Texas 762031 (A.
Plant Physiol ; 167(4): 1541-53, 2015 Apr.
Article en En | MEDLINE | ID: mdl-25681328
ABSTRACT
Phloem loading is a critical process in plant physiology. The potential of regulating the translocation of photoassimilates from source to sink tissues represents an opportunity to increase crop yield. Pyrophosphate homeostasis is crucial for normal phloem function in apoplasmic loaders. The involvement of Arabidopsis (Arabidopsis thaliana) type I proton-pumping pyrophosphatase (AVP1) in phloem loading was analyzed at genetic, histochemical, and physiological levels. A transcriptional AVP1 promoterGUS fusion revealed phloem activity in source leaves. Ubiquitous AVP1 overexpression (35SAVP1 cassette) enhanced shoot biomass, photoassimilate production and transport, rhizosphere acidification, and expression of sugar-induced root ion transporter genes (POTASSIUM TRANSPORTER2 [KUP2], NITRATE TRANSPORTER2.1 [NRT2.1], NRT2.4, and PHOSPHATE TRANSPORTER1.4 [PHT1.4]). Phloem-specific AVP1 overexpression (Commelina Yellow Mottle Virus promoter [pCOYMV]AVP1) elicited similar phenotypes. By contrast, phloem-specific AVP1 knockdown (pCoYMVRNAiAVP1) resulted in stunted seedlings in sucrose-deprived medium. We also present a promoter mutant avp1-2 (SALK046492) with a 70% reduction of expression that did not show severe growth impairment. Interestingly, AVP1 protein in this mutant is prominent in the phloem. Moreover, expression of an Escherichia coli-soluble pyrophosphatase in the phloem (pCoYMVpyrophosphatase) of avp1-2 plants resulted in severe dwarf phenotype and abnormal leaf morphology. We conclude that the Proton-Pumping Pyrophosphatase AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Arabidopsis / Difosfatos / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Pirofosfatasa Inorgánica Idioma: En Revista: Plant Physiol Año: 2015 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Arabidopsis / Difosfatos / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Pirofosfatasa Inorgánica Idioma: En Revista: Plant Physiol Año: 2015 Tipo del documento: Article