Visualizing the interior architecture of focal adhesions with high-resolution traction maps.
Nano Lett
; 15(4): 2220-8, 2015 Apr 08.
Article
en En
| MEDLINE
| ID: mdl-25730141
ABSTRACT
Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize traction forces within FAs with <100 nm spatial resolution. We find that αvß3 integrin selectively localizes to high force regions. Paxillin, which is not generally considered to play a direct role in force transmission, shows a higher degree of spatial correlation with force than vinculin, talin, or α-actinin, proteins with hypothesized roles as force transducers. These observations suggest that αvß3 integrin and paxillin may play important roles in mechanotransduction.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Adhesiones Focales
/
Proteínas del Citoesqueleto
/
Mecanotransducción Celular
/
Imagen Molecular
/
Proteínas de la Membrana
/
Microscopía Fluorescente
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nano Lett
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos