Choice of PCR microtube can impact on the success of long-range PCRs.
Anal Biochem
; 477: 115-7, 2015 May 15.
Article
en En
| MEDLINE
| ID: mdl-25766577
ABSTRACT
We compared four brands of microtubes with respect to their suitability for long-range polymerase chain reactions (PCRs). One of the four brands was found to have an inhibitory effect, decreasing PCR yields. The effect was universal across different PCR or enzyme systems. Increased ultraviolet absorbance suggests leaching of unknown chemical species into PCR mixtures. However, this could not be confirmed by high-performance liquid chromatography-mass spectrometry analysis. Nevertheless, our article demonstrates a clear impact of the choice of microtubes on long-range PCR success. Due consideration should be given to the PCR microtubes when determining optimal reaction conditions for long-range PCR.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Reacción en Cadena de la Polimerasa
Idioma:
En
Revista:
Anal Biochem
Año:
2015
Tipo del documento:
Article
País de afiliación:
Malasia