Inhibition of an NAD⺠salvage pathway provides efficient and selective toxicity to human pluripotent stem cells.
Stem Cells Transl Med
; 4(5): 483-93, 2015 May.
Article
en En
| MEDLINE
| ID: mdl-25834119
ABSTRACT
The tumorigenic potential of human pluripotent stem cells (hPSCs) is a major limitation to the widespread use of hPSC derivatives in the clinic. Here, we demonstrate that the small molecule STF-31 is effective at eliminating undifferentiated hPSCs across a broad range of cell culture conditions with important advantages over previously described methods that target metabolic processes. Although STF-31 was originally described as an inhibitor of glucose transporter 1, these data support the reclassification of STF-31 as a specific NAD⺠salvage pathway inhibitor through the inhibition of nicotinamide phosphoribosyltransferase (NAMPT). These findings demonstrate the importance of an NAD⺠salvage pathway in hPSC biology and describe how inhibition of NAMPT can effectively eliminate hPSCs from culture. These results will advance and accelerate the development of safe, clinically relevant hPSC-derived cell-based therapies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Piridinas
/
Diferenciación Celular
/
Células Madre Pluripotentes
/
NAD
Límite:
Humans
Idioma:
En
Revista:
Stem Cells Transl Med
Año:
2015
Tipo del documento:
Article
País de afiliación:
Liechtenstein