Virulent Burkholderia species mimic host actin polymerases to drive actin-based motility.
Cell
; 161(2): 348-60, 2015 Apr 09.
Article
en En
| MEDLINE
| ID: mdl-25860613
ABSTRACT
Burkholderia pseudomallei and B. mallei are bacterial pathogens that cause melioidosis and glanders, whereas their close relative B. thailandensis is non-pathogenic. All use the trimeric autotransporter BimA to facilitate actin-based motility, host cell fusion, and dissemination. Here, we show that BimA orthologs mimic different host actin-polymerizing proteins. B. thailandensis BimA activates the host Arp2/3 complex. In contrast, B. pseudomallei and B. mallei BimA mimic host Ena/VASP actin polymerases in their ability to nucleate, elongate, and bundle filaments by associating with barbed ends, as well as in their use of WH2 motifs and oligomerization for activity. Mechanistic differences among BimA orthologs resulted in distinct actin filament organization and motility parameters, which affected the efficiency of cell fusion during infection. Our results identify bacterial Ena/VASP mimics and reveal that pathogens imitate the full spectrum of host actin-polymerizing pathways, suggesting that mimicry of different polymerization mechanisms influences key parameters of infection.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
/
Moléculas de Adhesión Celular
/
Actinas
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Infecciones por Burkholderia
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Burkholderia
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Proteínas de Microfilamentos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cell
Año:
2015
Tipo del documento:
Article