Distinct adhesion-independent functions of ß-catenin control stage-specific sensory neurogenesis and proliferation.
BMC Biol
; 13: 24, 2015 Apr 11.
Article
en En
| MEDLINE
| ID: mdl-25885041
ABSTRACT
BACKGROUND:
ß-catenin plays a central role in multiple developmental processes. However, it has been difficult to study its pleiotropic effects, because of the dual capacity of ß-catenin to coordinate cadherin-dependent cell adhesion and to act as a component of Wnt signal transduction. To distinguish between the divergent functions of ß-catenin during peripheral nervous system development, we made use of a mutant allele of ß-catenin that can mediate adhesion but not Wnt-induced TCF transcriptional activation. This allele was combined with various conditional inactivation approaches.RESULTS:
We show that of all peripheral nervous system structures, only sensory dorsal root ganglia require ß-catenin for proper formation and growth. Surprisingly, however, dorsal root ganglia development is independent of cadherin-mediated cell adhesion. Rather, both progenitor cell proliferation and fate specification are controlled by ß-catenin signaling. These can be divided into temporally sequential processes, each of which depends on a different function of ß-catenin.CONCLUSIONS:
While early stage proliferation and specific Neurog2- and Krox20-dependent waves of neuronal subtype specification involve activation of TCF transcription, late stage progenitor proliferation and Neurog1-marked sensory neurogenesis are regulated by a function of ß-catenin independent of TCF activation and adhesion. Thus, switching modes of ß-catenin function are associated with consecutive cell fate specification and stage-specific progenitor proliferation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Receptoras Sensoriales
/
Beta Catenina
/
Neurogénesis
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
BMC Biol
Asunto de la revista:
BIOLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Suiza