Comparison of two methods for the detection of verotoxin producing E.coli in human faecal samples during an outbreak of HUS in Apulia, Southern Italy.
New Microbiol
; 38(2): 277-80, 2015 Apr.
Article
en En
| MEDLINE
| ID: mdl-25938754
ABSTRACT
This study evaluated the diagnostic performances of an ELISA method and a molecular method for the detection of verotoxin in faecal samples during an outbreak of haemolytic-uraemic syndrome (HUS) occurring in Apulia, Southern Italy. Two of the 16 faecal samples were positive for verotoxin when analysed by ELISA and resulted PCR positive for stx1, stx2, eaeA and serogroup O26. The other 14 faecal samples resulted negative with both tests. The detection of verotoxin in faecal samples by ELISA is a simple, sensitive, specific and rapid method (2 hours) of considerable utility for routine clinical testing laboratories without access to more specialized diagnostic procedures.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Reacción en Cadena de la Polimerasa
/
Toxinas Shiga
/
Escherichia coli
/
Infecciones por Escherichia coli
/
Heces
/
Síndrome Hemolítico-Urémico
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
País/Región como asunto:
Europa
Idioma:
En
Revista:
New Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Italia