NGL-2 Is a New Partner of PAR Complex in Axon Differentiation.
J Neurosci
; 35(18): 7153-64, 2015 May 06.
Article
en En
| MEDLINE
| ID: mdl-25948265
ABSTRACT
Neuronal polarization is pivotal for neural network formation during brain development. Axon differentiation is a hallmark of initial neuronal polarization. Here, we report that the leucine-rich repeat-containing protein netrin-G ligand-2 (NGL-2) as a polarity regulator that localizes asymmetrically in rat hippocampal neurons and is required for differentiation of the future axon. NGL-2 was associated with PAR complex, and this interaction resulted in local stabilization of axonal microtubules. Further study showed that the C terminal of NGL-2 binds to the PDZ domain of PAR6, and NGL-2 interacts with PAR3 and atypical PKCζ (aPKCζ), with PAR6 acting as a bridge or modifier. Then, NGL-2 regulates the local stabilization of microtubules and promotes axon differentiation by the aPKCζ/microtubule affinity-regulating kinase 2 pathway. These findings reveal the critical role of NGL-2 in regulating axon differentiation in rat hippocampal neurons and reveal a novel partner of the PAR complex.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Axones
/
Proteínas Portadoras
/
Diferenciación Celular
/
Proteínas Ligadas a GPI
/
Proteínas del Tejido Nervioso
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Neurosci
Año:
2015
Tipo del documento:
Article