Determination of mannitol sorbitol and myo-inositol in olive tree roots and rhizospheric soil by gas chromatography and effect of severe drought conditions on their profiles.
J Chromatogr Sci
; 53(10): 1631-8, 2015.
Article
en En
| MEDLINE
| ID: mdl-26045583
ABSTRACT
This study reports a method for the analysis of mannitol, sorbitol and myo-inositol in olive tree roots and rhizospheric soil with gas chromatography. The analytical method consists of extraction with a mixture of dichloromethanemethanol (21, v/v) for soil samples and a mixture of ethanolwater (8020) for root samples, silylation using pyridine, hexamethyldisilazane (HMDS) and trimethylchlorosilane (TMCS). The recovery of mannitol sorbitol and myo-inositol (for extraction and analysis in dichloromethanemethanol and ethanolwater) was acceptable and ranged from 100.3 to 114.7%. The time of analysis was <24 min. Among identified polyols extracted from rhizosphere and roots of olive plants, mannitol was the major compound. A marked increase in mannitol content occurred in rhizosphere and roots of water-stressed plants, suggesting a much broader role of mannitol in stress response based on its ability to act as a compatible solute.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sorbitol
/
Cromatografía de Gases
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Raíces de Plantas
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Olea
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Sequías
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Inositol
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Manitol
Idioma:
En
Revista:
J Chromatogr Sci
Año:
2015
Tipo del documento:
Article
País de afiliación:
Túnez