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Cutting Edge: A Natural Antisense Transcript, AS-IL1α, Controls Inducible Transcription of the Proinflammatory Cytokine IL-1α.
Chan, Jennie; Atianand, Maninjay; Jiang, Zhaozhao; Carpenter, Susan; Aiello, Daniel; Elling, Roland; Fitzgerald, Katherine A; Caffrey, Daniel R.
Afiliación
  • Chan J; Program in Innate Immunity, Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605;
  • Atianand M; Program in Innate Immunity, Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605;
  • Jiang Z; Program in Innate Immunity, Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605;
  • Carpenter S; Program in Innate Immunity, Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605; Department of Immunology and Microbiology, University of California, San Francisco, San Francisco, CA 94143; and.
  • Aiello D; Program in Innate Immunity, Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605;
  • Elling R; Program in Innate Immunity, Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605;
  • Fitzgerald KA; Program in Innate Immunity, Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605; Centre for Molecular Inflammation Research, Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, 7491
  • Caffrey DR; Program in Innate Immunity, Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605; Kate.Fitzgerald@umassmed.edu Daniel.Caffrey@umassmed.edu.
J Immunol ; 195(4): 1359-63, 2015 Aug 15.
Article en En | MEDLINE | ID: mdl-26179904
Natural antisense transcripts (NATs) are a class of long noncoding RNAs (lncRNAs) that are complementary to other protein-coding genes. Although thousands of NATs are encoded by mammalian genomes, their functions in innate immunity are unknown. In this study, we identified and characterized a novel NAT, AS-IL1α, which is partially complementary to IL-1α. Similar to IL-1α, AS-IL1α is expressed at low levels in resting macrophages and is induced following infection with Listeria monocytogenes or stimulation with TLR ligands (Pam3CSK4, LPS, polyinosinic-polycytidylic acid). Inducible expression of IL-1α mRNA and protein were significantly reduced in macrophages expressing shRNA that target AS-IL1α. AS-IL1α is located in the nucleus and did not alter the stability of IL-1α mRNA. Instead, AS-IL1α was required for the recruitment of RNA polymerase II to the IL-1α promoter. In summary, our studies identify AS-IL1α as an important regulator of IL-1α transcription during the innate immune response.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Transcripción Genética / Regulación de la Expresión Génica / ARN sin Sentido / Mediadores de Inflamación / ARN no Traducido / Interleucina-1alfa Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Immunol Año: 2015 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Transcripción Genética / Regulación de la Expresión Génica / ARN sin Sentido / Mediadores de Inflamación / ARN no Traducido / Interleucina-1alfa Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Immunol Año: 2015 Tipo del documento: Article