Composition and variation analysis of the TCR ß-chain CDR3 repertoire in systemic lupus erythematosus using high-throughput sequencing.
Mol Immunol
; 67(2 Pt B): 455-64, 2015 Oct.
Article
en En
| MEDLINE
| ID: mdl-26227771
ABSTRACT
The ability of T lymphocytes to mount an immune response against a diverse array of pathogens is primarily conveyed by the amino acid (aa) sequence of the hypervariable complementarity-determining region 3 (CDR3) segments of the T cell receptor (TCR). In this study, we used a combination of multiplex-PCR, Illumina sequencing and IMGT/HighV-QUEST for a standardized analysis of the characteristics and polymorphisms of the T-cell receptor BV complementarity-determining region 3 (TCR BV CDR3) gene in peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy donors (NC). We found the distributions of CDR3, VD indel, and DJ indel lengths to be comparable between the SLE and NC groups. The degree of clonal expansion in the SLE group was significantly greater than in the NC group, and the expression levels of 10 TRßV segments and 6 TRßJ segments were also significantly different in the SLE group. Regarding public T cell responses, 3CDR3 DNA sequences and 4 aa sequences were shared by all SLE patients and may serve as biomarkers for SLE disease risk, diagnosis and/or prognosis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Variación Genética
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Receptores de Antígenos de Linfocitos T alfa-beta
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Regiones Determinantes de Complementariedad
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Secuenciación de Nucleótidos de Alto Rendimiento
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Lupus Eritematoso Sistémico
Tipo de estudio:
Observational_studies
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Prognostic_studies
/
Risk_factors_studies
Límite:
Adult
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Mol Immunol
Año:
2015
Tipo del documento:
Article
País de afiliación:
China