[Apoptosis in Lungs and Liver after Crush Injury of Hindlimbs in Rat].
Fa Yi Xue Za Zhi
; 31(2): 88-92, 2015 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-26245082
ABSTRACT
OBJECTIVE:
To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanism of crush injury.METHODS:
The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry.RESULTS:
Compared with the control group, the partial muscle injury of rat's hindlimbs was more serious with more apoptosis observed in lungs and liver (P < 0.05). The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated (P < 0.05).CONCLUSION:
The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The correlation factor released during tissue injury may mediate apoptosis process.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Apoptosis
/
Caspasa 3
/
Miembro Posterior
/
Hígado
/
Pulmón
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Fa Yi Xue Za Zhi
Asunto de la revista:
JURISPRUDENCIA
Año:
2015
Tipo del documento:
Article