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Regulation of Nicotine Biosynthesis by an Endogenous Target Mimicry of MicroRNA in Tobacco.
Li, Fangfang; Wang, Weidi; Zhao, Nan; Xiao, Bingguang; Cao, Peijian; Wu, Xingfu; Ye, Chuyu; Shen, Enhui; Qiu, Jie; Zhu, Qian-Hao; Xie, Jiahua; Zhou, Xueping; Fan, Longjiang.
Afiliación
  • Li F; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Wang W; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Zhao N; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Xiao B; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Cao P; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Wu X; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Ye C; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Shen E; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Qiu J; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Zhu QH; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Xie J; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Zhou X; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
  • Fan L; Institute of Crop Science and Research Center for Air Pollution and Health (F.L., W.W., N.Z., C.Y., E.S., J.Q., L.F.) and Institute of Biotechnology (F.L., N.Z., X.Z.), Zhejiang University, Hangzhou 310058, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Cen
Plant Physiol ; 169(2): 1062-71, 2015 Oct.
Article en En | MEDLINE | ID: mdl-26246450
ABSTRACT
The interaction between noncoding endogenous target mimicry (eTM) and its corresponding microRNA (miRNA) is a newly discovered regulatory mechanism and plays pivotal roles in various biological processes in plants. Tobacco (Nicotiana tabacum) is a model plant for studying secondary metabolite alkaloids, of which nicotine accounts for approximately 90%. In this work, we identified four unique tobacco-specific miRNAs that were predicted to target key genes of the nicotine biosynthesis and catabolism pathways and an eTM, novel tobacco miRNA (nta)-eTMX27, for nta-miRX27 that targets QUINOLINATE PHOSPHORIBOSYLTRANSFERASE2 (QPT2) encoding a quinolinate phosphoribosyltransferase. The expression level of nta-miRX27 was significantly down-regulated, while that of QPT2 and nta-eTMX27 was significantly up-regulated after topping, and consequently, nicotine content increased in the topping-treated plants. The topping-induced down-regulation of nta-miRX27 and up-regulation of QPT2 were only observed in plants with a functional nta-eTMX27 but not in transgenic plants containing an RNA interference construct targeting nta-eTMX27. Our results demonstrated that enhanced nicotine biosynthesis in the topping-treated tobacco plants is achieved by nta-eTMX27-mediated inhibition of the expression and functions of nta-miRX27. To our knowledge, this is the first report about regulation of secondary metabolite biosynthesis by an miRNA-eTM regulatory module in plants.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Nicotiana / MicroARNs / Nicotina Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2015 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Nicotiana / MicroARNs / Nicotina Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2015 Tipo del documento: Article