A novel terpenoid indole alkaloid derived from catharanthine via biotransformation by suspension-cultured cells of Catharanthus roseus.
Biotechnol Lett
; 37(12): 2481-7, 2015 Dec.
Article
en En
| MEDLINE
| ID: mdl-26272394
ABSTRACT
OBJECTIVE:
Although catharanthine (1) is well known as a biosynthetic precursor of the anticancer alkaloid, vinblastine, its alternative metabolic pathways are unclear.RESULTS:
Biotransformation of 1 by suspension-cultured cells of Catharanthus roseus gave a new oxidative-cleavage product (2). The structure of 2 was determined as 3-hydroxy-4-imino-catharanthine by spectroscopic methods. Maximum conversion (9.75 %) of 2 was observed after 120 h adding 6 mg of 1/100 ml to 12-day-old suspension-cultured cells of C. roseus. Furthermore, qRT-PCR experiment was performed to reveal the effect of 1 on the expression of the genes in the biosynthetic pathway of TIA 1 up-regulated the transcript level of D4H whilst down-regulating the transcript levels of G10H, LAMT, GES, and IRS.CONCLUSION:
A new metabolite of catharanthine, 3-hydroxy-4-imino-catharanthine, is reported.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Alcaloides de la Vinca
/
Catharanthus
/
Alcaloides de Triptamina Secologanina
Idioma:
En
Revista:
Biotechnol Lett
Año:
2015
Tipo del documento:
Article
País de afiliación:
China