Riboflavin and ultraviolet A as adjuvant treatment against Acanthamoeba cysts.
Clin Exp Ophthalmol
; 44(3): 181-7, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-26355273
ABSTRACT
BACKGROUND:
Experimental studies have shown that the standard dose of riboflavin (R) or R + ultraviolet-A (UVA) as solo treatment are not able to exterminate Acanthamoeba cysts or even trophozoites. The purpose of this study is to determine whether the application of R + UVA can enhance the cysticidal effects of cationic antiseptic agents in vitro.METHODS:
The log of either polyhexamethylene biguanide or chlorhexidine minimal cysticidal concentration in solutions containing riboflavin (concentrations 0.1, 0.05 and 0.025%) plus either Acanthamoeba castellanii cysts or Acanthamoeba polyphaga cysts was determined and compared in groups treated with UVA 30 mW/cm(2) for 30 min and in control groups (with no exposure to UVA). A permutation test was used to determine the P value associated with treatment.RESULTS:
Regardless of the riboflavin concentration and UVA treatment condition, no trophozoites were seen in plates where the cysts were previously exposed to cationic antiseptic agent concentrations ≥200 µg/mL for Acanthamoeba castellanii samples and ≥100 µg/mL for A. polyphaga samples. There was no statistical evidence that R + UVA treatment was associated with minimal cysticidal concentration (P = 0.82).CONCLUSION:
R + UVA in doses up to 10 times higher than recommended for corneal crosslinking does not enhance the cysticidal effect of either polyhexamethylene biguanide or chlorhexidine in vitro.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fotoquimioterapia
/
Riboflavina
/
Rayos Ultravioleta
/
Acanthamoeba
/
Fármacos Fotosensibilizantes
/
Desinfectantes
Límite:
Humans
Idioma:
En
Revista:
Clin Exp Ophthalmol
Asunto de la revista:
OFTALMOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos