Spatial light modulator phase mask implementation of wavefront encoded 3D computational-optical microscopy.
Appl Opt
; 54(29): 8587-95, 2015 Oct 10.
Article
en En
| MEDLINE
| ID: mdl-26479791
ABSTRACT
Spatial light modulator (SLM) implementation of wavefront encoding enables various types of engineered point-spread functions (PSFs), including the generalized-cubic and squared-cubic phase mask wavefront encoded (WFE) PSFs, shown to reduce the impact of sample-induced spherical aberration in fluorescence microscopy. This investigation validates dynamic experimental parameter variation of these WFE-PSFs. We find that particular design parameter bounds exist, within which the divergence of computed and experimental WFE-PSFs is of the same order of magnitude as that of computed and experimental conventional PSFs, such that model-based approaches for solving the inverse imaging problem can be applied to a wide range of SLM-WFE systems. Interferometric measurements were obtained to evaluate the SLM implementation of the numeric mask. Agreement between experiment and theory in terms of a wrapped phase, 0-2π, validates the phase mask implementation and allows characterization of the SLM response. These measurements substantiate experimental practice of computational-optical microscope imaging with an SLM-engineered PSF.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
Appl Opt
Año:
2015
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Article