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Caspase-3 cleaved p65 fragment dampens NF-κB-mediated anti-apoptotic transcription by interfering with the p65/RPS3 interaction.
Wier, Eric M; Fu, Kai; Hodgson, Andrea; Sun, Xin; Wan, Fengyi.
Afiliación
  • Wier EM; Department of Biochemistry and Molecular Biology, USA.
  • Fu K; Department of Biochemistry and Molecular Biology, USA.
  • Hodgson A; Department of Biochemistry and Molecular Biology, USA; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21025, USA.
  • Sun X; Department of Biochemistry and Molecular Biology, USA.
  • Wan F; Department of Biochemistry and Molecular Biology, USA; Department of Oncology, Johns Hopkins University, Baltimore, MD 21287, USA; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA. Electronic address: fwan1@jhu.edu.
FEBS Lett ; 589(23): 3581-7, 2015 Nov 30.
Article en En | MEDLINE | ID: mdl-26526615
Caspase-3-mediated p65 cleavage is believed to suppress nuclear factor-kappa B (NF-κB)-mediated anti-apoptotic transactivation in cells undergoing apoptosis. However, only a small percentage of p65 is cleaved during apoptosis, not in proportion to the dramatic reduction in NF-κB transactivation. Here we show that the p65(1-97) fragment generated by Caspase-3 cleavage interferes with ribosomal protein S3 (RPS3), an NF-κB "specifier" subunit, and selectively retards the nuclear translocation of RPS3, thus dampening the RPS3/NF-κB-dependent anti-apoptotic gene expression. Our findings reveal a novel cell fate determination mechanism to ensure cells undergo programed cell death through interfering with RPS3/NF-κB-conferred anti-apoptotic transcription by the fragment from partial p65 cleavage by activated Caspase-3.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fragmentos de Péptidos / Proteínas Ribosómicas / Transcripción Genética / Apoptosis / Factor de Transcripción ReIA / Caspasa 3 / Proteolisis Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: FEBS Lett Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fragmentos de Péptidos / Proteínas Ribosómicas / Transcripción Genética / Apoptosis / Factor de Transcripción ReIA / Caspasa 3 / Proteolisis Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: FEBS Lett Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos