Host microbiota modulates development of social preference in mice.
Microb Ecol Health Dis
; 26: 29719, 2015.
Article
en En
| MEDLINE
| ID: mdl-26679775
ABSTRACT
BACKGROUND:
Mounting evidence indicates that the indigenous gut microbiota exerts long-lasting programming effects on brain function and behaviour.OBJECTIVE:
In this study, we used the germ-free (GF) mouse model, devoid of any microbiota throughout development, to assess the influence of the indigenous microbiota on social preference and repetitive behaviours (e.g. self-grooming). METHODS ANDRESULTS:
Using the three-chambered social approach task, we demonstrate that when adult GF mice were given a choice to spend time with a novel mouse or object, they spent significantly more time sniffing and interacting with the stimulus mouse compared to conventionally raised mice (specific pathogen-free, SPF). Time spent in repetitive self-grooming behaviour, however, did not differ between GF and SPF mice. Real-time PCR-based gene expression analysis of the amygdala, a key region that is part of the social brain network, revealed a significant reduction in the mRNA levels of total brain-derived neurotrophic factor (BDNF), BDNF exon I-, IV-, VI-, IX-containing transcripts, and NGFI-A (a signalling molecule downstream of BDNF) in GF mice compared to SPF mice.CONCLUSION:
These results suggest that differential regulation of BDNF exon transcripts in the amygdala by the indigenous microbes may contribute to the altered social development of GF mice.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Microb Ecol Health Dis
Año:
2015
Tipo del documento:
Article
País de afiliación:
Suecia