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S1P3 receptor influences key physiological properties of fast-twitch extensor digitorum longus muscle.
Germinario, Elena; Bondì, Michela; Cencetti, Francesca; Donati, Chiara; Nocella, Marta; Colombini, Barbara; Betto, Romeo; Bruni, Paola; Bagni, Maria Angela; Danieli-Betto, Daniela.
Afiliación
  • Germinario E; Department of Biomedical Sciences, University of Padova, Padova, Italy; IIM, Interuniversity Institute of Myology, Italy;
  • Bondì M; Department of Biomedical Sciences, University of Padova, Padova, Italy;
  • Cencetti F; IIM, Interuniversity Institute of Myology, Italy; Department of Biomedical, Experimental and Clinical Sciences, Mario Serio, University of Firenze, Florence, Italy;
  • Donati C; IIM, Interuniversity Institute of Myology, Italy; Department of Biomedical, Experimental and Clinical Sciences, Mario Serio, University of Firenze, Florence, Italy;
  • Nocella M; IIM, Interuniversity Institute of Myology, Italy; Department of Experimental and Clinical Medicine, University of Firenze, Florence, Italy;
  • Colombini B; IIM, Interuniversity Institute of Myology, Italy; Department of Experimental and Clinical Medicine, University of Firenze, Florence, Italy;
  • Betto R; IIM, Interuniversity Institute of Myology, Italy; CNR-Institute for Neuroscience, CNR, Padova, Italy.
  • Bruni P; IIM, Interuniversity Institute of Myology, Italy; Department of Biomedical, Experimental and Clinical Sciences, Mario Serio, University of Firenze, Florence, Italy;
  • Bagni MA; IIM, Interuniversity Institute of Myology, Italy; Department of Experimental and Clinical Medicine, University of Firenze, Florence, Italy;
  • Danieli-Betto D; Department of Biomedical Sciences, University of Padova, Padova, Italy; IIM, Interuniversity Institute of Myology, Italy; daniela.danieli@unipd.it.
J Appl Physiol (1985) ; 120(11): 1288-300, 2016 Jun 01.
Article en En | MEDLINE | ID: mdl-26718782
To examine the role of sphingosine 1-phosphate (S1P) receptor 3 (S1P3) in modulating muscle properties, we utilized transgenic mice depleted of the receptor. Morphological analyses of extensor digitorum longus (EDL) muscle did not show evident differences between wild-type and S1P3-null mice. The body weight of 3-mo-old S1P3-null mice and the mean cross-sectional area of transgenic EDL muscle fibers were similar to those of wild-type. S1P3 deficiency enhanced the expression level of S1P1 and S1P2 receptors mRNA in S1P3-null EDL muscle. The contractile properties of S1P3-null EDL diverge from those of wild-type, largely more fatigable and less able to recover. The absence of S1P3 appears responsible for a lower availability of calcium during fatigue. S1P supplementation, expected to stimulate residual S1P receptors and signaling, reduced fatigue development of S1P3-null muscle. Moreover, in the absence of S1P3, denervated EDL atrophies less than wild-type. The analysis of atrophy-related proteins in S1P3-null EDL evidences high levels of the endogenous regulator of mitochondria biogenesis peroxisome proliferative-activated receptor-γ coactivator 1α (PGC-1α); preserving mitochondria could protect the muscle from disuse atrophy. In conclusion, the absence of S1P3 makes the muscle more sensitive to fatigue and slows down atrophy development after denervation, indicating that S1P3 is involved in the modulation of key physiological properties of the fast-twitch EDL muscle.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fibras Musculares de Contracción Rápida / Receptores de Lisoesfingolípidos Límite: Animals Idioma: En Revista: J Appl Physiol (1985) Asunto de la revista: FISIOLOGIA Año: 2016 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fibras Musculares de Contracción Rápida / Receptores de Lisoesfingolípidos Límite: Animals Idioma: En Revista: J Appl Physiol (1985) Asunto de la revista: FISIOLOGIA Año: 2016 Tipo del documento: Article