Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans.
Anal Biochem
; 501: 1-3, 2016 May 15.
Article
en En
| MEDLINE
| ID: mdl-26876105
ABSTRACT
Mannose-6-phosphate (M-6-P) glycan analysis is important for quality control of therapeutic enzymes for lysosomal storage diseases. Here, we found that the analysis of glycans containing two M-6-Ps was highly affected by the hydrophilicity of the elution solvent used in high-performance liquid chromatography (HPLC). In addition, the performances of three fluorescent tags--2-aminobenzoic acid (2-AA), 2-aminobenzamide (2-AB), and 3-(acetyl-amino)-6-aminoacridine (AA-Ac)--were compared with each other for M-6-P glycan analysis using HPLC and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The best performance for analyzing M-6-P glycans was shown by 2-AA labeling in both analyses.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Colorantes Fluorescentes
/
Manosafosfatos
Idioma:
En
Revista:
Anal Biochem
Año:
2016
Tipo del documento:
Article