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Ploidy effect and genetic architecture exploration of stalk traits using DH and its corresponding haploid populations in maize.
Meng, Yujie; Li, Junhui; Liu, Jianju; Hu, Haixiao; Li, Wei; Liu, Wenxin; Chen, Shaojiang.
Afiliación
  • Meng Y; National Maize Improvement Center of China, China Agricultural University (West Campus), 2# Yuanmingyuan West Road, Beijing, 100193, China. mengyujie25@126.com.
  • Li J; Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy, China Agricultural University (West Campus), 2# Yuanmingyuan West Road, Beijing, 100193, China. junhuili@cau.edu.cn.
  • Liu J; National Maize Improvement Center of China, China Agricultural University (West Campus), 2# Yuanmingyuan West Road, Beijing, 100193, China. jianjuliu23@163.com.
  • Hu H; Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, 70599, Stuttgart, Germany. huganye@163.com.
  • Li W; National Maize Improvement Center of China, China Agricultural University (West Campus), 2# Yuanmingyuan West Road, Beijing, 100193, China. wellion@cau.edu.cn.
  • Liu W; National Maize Improvement Center of China, China Agricultural University (West Campus), 2# Yuanmingyuan West Road, Beijing, 100193, China. wenxinliu@cau.edu.cn.
  • Chen S; Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy, China Agricultural University (West Campus), 2# Yuanmingyuan West Road, Beijing, 100193, China. wenxinliu@cau.edu.cn.
BMC Plant Biol ; 16: 50, 2016 Feb 25.
Article en En | MEDLINE | ID: mdl-26911156
ABSTRACT

BACKGROUND:

Doubled haploid (DH) lines produced via in vivo haploid induction have become indispensable in maize research and practical breeding, so it is important to understand traits characteristics in DH and its corresponding haploids which derived from each DH lines. In this study, a DH population derived from Zheng58 × Chang7-2 and a haploid population, were developed, genotyped and evaluated to investigate genetic architecture of eight stalk traits, especially rind penetrometer resistance (RPR) and in vitro dry matter digestion (IVDMD), which affecting maize stalk lodging-resistance and feeding values, respectively.

RESULTS:

Phenotypic correlation coefficients ranged from 0.38 to 0.69 between the two populations for eight stalk traits. Heritability values of all stalk traits ranged from 0.49 to 0.81 in the DH population, and 0.58 to 0.89 in the haploid population. Quantitative trait loci (QTL) mapping study showed that a total of 47 QTL for all traits accounting for genetic variations ranging from 1.6 to 36.5% were detected in two populations. One or more QTL sharing common region for each trait were detected between two different ploidy populations. Potential candidate genes predicated from the four QTL support intervals for RPR and IVDMD were indirectly or directly involved with cellulose and lignin biosynthesis, which participated in cell wall formation. The increased expression levels of lignin and cellulose synthesis key genes in the haploid situation illustrated that dosage compensation may account for genome dosage effect in our study.

CONCLUSIONS:

The current investigation extended understanding about the genetic basis of stalk traits and correlations between DH and its haploid populations, which showed consistence and difference between them in phenotype, QTL characters, and gene expression. The higher heritabilities and partly higher QTL detection power were presented in haploid population than in DH population. All of which described above could lay a preliminary foundation for genetic architecture study with haploid population and may benefit selection in haploid-stage to reduce cost in DH breeding.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tallos de la Planta / Zea mays / Haploidia Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2016 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tallos de la Planta / Zea mays / Haploidia Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2016 Tipo del documento: Article País de afiliación: China