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A SIMPLE AND EFFICIENT VITRIFICATION METHOD FOR IN-STRAW DILUTION AND DIRECT TRANSFER OF BOVINE EMBRYOS.
Zhang, Youwen; Fu, Xiangwei; Chen, Long; Feng, Chuntao; Bi, Jianghua; Mo, Xianhong; Cheng, Keren; Zhang, Rina; Li, Shujing; Zhu, Shien.
Afiliación
  • Zhang Y; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
  • Fu X; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
  • Chen L; Bingjing AnBo Embryo Biotech Center, Beijing, China.
  • Feng C; Bingjing AnBo Embryo Biotech Center, Beijing, China.
  • Bi J; Bingjing AnBo Embryo Biotech Center, Beijing, China.
  • Mo X; College of Biological Sciences, China Agricultural University, Beijing, China.
  • Cheng K; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
  • Zhang R; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
  • Li S; Bingjing AnBo Embryo Biotech Center, Beijing, China.
  • Zhu S; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. zhushien@cau.edu.cn.
Cryo Letters ; 36(6): 392-8, 2015.
Article en En | MEDLINE | ID: mdl-26963885
ABSTRACT

BACKGROUND:

An easy and user friendly protocol that produces consistent results will facilitate the commercial application of embryo vitrification technology in the field.

OBJECTIVE:

This study was designed to develop a simple and efficient vitrification, in-straw dilution and direct transfer method for bovine embryos.

METHODS:

After being vitrified and in-straw thawed, in vivo-derived and in vitro-produced bovine embryos were subjected to in vitro culture or embryo transplantation.

RESULTS:

There were no significant differences (P > 0.05) in survival rates (100.0% vs. 93.9%) and expansion rates (93.8% vs. 87.5%) between in vivo-derived and in vitro-produced blastocysts after vitrification and in-straw dilution. And there was also no significant difference (P > 0.05) in conception rates (56.5% vs. 58.8%) after ET between cryopreserved and fresh in vivo-derived blastocysts.

CONCLUSION:

Vitrification using EG-based vitrification solution and in-straw dilution with PBS-based diluent is a simple and efficient method for cryopreservation and direct transfer of bovine embryos.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fertilización In Vitro / Criopreservación / Transferencia de Embrión / Vitrificación Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: China
Buscar en Google
Imprimir
XML
PubMed Links

Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fertilización In Vitro / Criopreservación / Transferencia de Embrión / Vitrificación Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: China