The Association Between WAVE1 and -3 and the ARP2/3 Complex in PC 3 Cells.
Anticancer Res
; 36(3): 1135-42, 2016 Mar.
Article
en En
| MEDLINE
| ID: mdl-26977009
ABSTRACT
BACKGROUND:
Actin polymerisation is stimulated by the actin-related protein (ARP) 2/3 complex and drives cell migration. This complex is activated by Wiskott-Aldrich syndrome protein family (WASP) verprolin homologous protein (WAVE) proteins. WAVE1 and -3 have been implicated in the aggressiveness of metastatic prostate cancer cells. MATERIALS ANDMETHODS:
Cell growth, motility and invasion were analyzed in WAVE1- and WAVE3-knockdown PC-3 cells along with the ARP2/3 inhibitor, CK-0944636. Confocal microscopy was adopted to examine protein co-localisation. Immunoprecipitation approaches were used to determine protein tyrosine phosphorylation.RESULTS:
Cell growth suppression was observed with WAVE3 knockdown and ARP2/3 inhibition. Reduced cell invasion effects observed with WAVE1 knockdown appeared to be rescued by ARP2/3 inhibition. WAVE1 and WAVE3 and ARP2 co-localisation was lost in PC-3 WAVE-knockdown cells, while increased ARP2 tyrosine phosphorylation was observed with WAVE3 knockdown.CONCLUSION:
These results implicate a contributory role of WAVE1 and -3 to the metastatic phenotype of PC-3 cells through their interaction with the ARP2/3 complex.Palabras clave
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
/
Citoesqueleto de Actina
/
Actinas
/
Complejo 2-3 Proteico Relacionado con la Actina
/
Familia de Proteínas del Síndrome de Wiskott-Aldrich
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
Anticancer Res
Año:
2016
Tipo del documento:
Article
País de afiliación:
Reino Unido