Toxicological interactions of silver nanoparticles and organochlorine pesticides in mouse peritoneal macrophages.
Toxicol Mech Methods
; 26(4): 251-9, 2016 May.
Article
en En
| MEDLINE
| ID: mdl-27001549
ABSTRACT
Nanotechnology occupies a prominent space in economy and science due to the beneficial properties of nanomaterials. However, nanoparticles may pose risks to living organisms due to their adsorption and pro-oxidative properties. The aim of the current study was to investigate the effects of polymer-coated silver nanoparticles (AgNPs) and organochlorine pesticides (OCPs), as well as their combined effects on mouse peritoneal macrophages. Macrophages were isolated and exposed to three concentrations of AgNPs (groups N1 = 30, N2 = 300 and N3 = 3000 ng.ml(-1)), two concentrations of OCPs (groups P1 = 30 and P2 = 300 ng.ml(-1)) and the six possible combinations of these two contaminants for 24 h. AgNPs had irregular shape, Feret diameter of 8.7 ± 7.5 nm and zeta potential of -28.7 ± 3.9 mV in water and -10.7 ± 1.04 mV in culture medium. OCP mixtures and the lower concentrations of AgNPs had no detectable effects on cell parameters, but the highest AgNPs concentration showed high toxicity (trypan blue and MTT assays) resulting in morphological changes, increase of nitric oxide levels and phagocytic index. Foremost, the association of N3 and P2 led to distinct effects from those observed under single exposure.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plaguicidas
/
Plata
/
Macrófagos Peritoneales
/
Nanopartículas del Metal
/
Hidrocarburos Clorados
Límite:
Animals
Idioma:
En
Revista:
Toxicol Mech Methods
Asunto de la revista:
TOXICOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Brasil