DESICCATION STRESS CAUSES STRUCTURAL AND ULTRASTRUCTURAL ALTERATIONS IN THE AEROTERRESTRIAL GREEN ALGA KLEBSORMIDIUM CRENULATUM (KLEBSORMIDIOPHYCEAE, STREPTOPHYTA) ISOLATED FROM AN ALPINE SOIL CRUST1.
J Phycol
; 47(3): 591-602, 2011 Jun.
Article
en En
| MEDLINE
| ID: mdl-27021989
ABSTRACT
Klebsormidium crenulatum (Kütz.) Lokhorst (Klebsormidiophyceae, Streptophyta) isolated from an alpine soil in Tyrol, Austria, was experimentally exposed to desiccation under various relative air humidities (RH 5, 75, and >95%, ambient air 55%-60%). The effects on the structure and ultrastructure of K. crenulatum after 1, 4, or 7 d of desiccation at 5, 75, and >95% RH were investigated. The cross walls were deformed to an undulated shape, and the cell diameter was reduced to â¼60% of the control. Regardless of the RH applied, in all cases the cytoplasm appeared denser compared to that of liquid-culture-grown cells. Electron-dense particles with diameters of 0.4 µm-0.8 µm were observed in the cytoplasm, likely representing lipid droplets. The chloroplasts of desiccated samples contained a large number of plastoglobules. The number and appearance of mitochondria were not visibly altered, as also verified by 3,3' dihexyloxacarbocyanine iodine (DIOC6 ) staining. The amphiphilic styryl dye FM 1-43 resulted in staining of the plasma membrane in cells from liquid culture. In 7 d desiccated samples, a marked fluorescence is seen in â¼40%-50% of the cells, which were dead. Actin microfilaments (MFs) were drastically disrupted after desiccation; only dotlike actin batches remained. These results demonstrate that flexibility of the cell walls and maintenance of the key organelles play a key role in the tolerance of desiccation stress in K. crenulatum.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Tipo de estudio:
Etiology_studies
Idioma:
En
Revista:
J Phycol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania