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Real-time monitoring of DNA methyltransferase activity using a hemimethylated smart probe.
Jin, Shunxin; Liu, Haisheng; Xia, Kun; Ma, Changbei; He, Hailun; Wang, Kemin.
Afiliación
  • Jin S; State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China.
  • Liu H; State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China.
  • Xia K; State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China.
  • Ma C; State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410081, China. Electronic address: macb2012@csu.edu.cn.
  • He H; State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China.
  • Wang K; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410081, China.
Mol Cell Probes ; 30(3): 185-7, 2016 06.
Article en En | MEDLINE | ID: mdl-27039360
A real-time assay for DNA methyltransferase (MTase) activity has been developed. A hemimethylated smart probe is used as the substrate for DNA MTase. Cleavage of the methylated product leads to separation of fluorophore from quencher, giving a proportional increase in fluorescence. The method permits real-time monitoring of DNA methylation process and makes it easy to characterize the activity of DNA MTase. It also has the potential to screen suitable inhibitor drugs for DNA MTase.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sistemas de Computación / Metiltransferasa de ADN de Sitio Específico (Adenina Especifica) / Sondas de ADN / Metilación de ADN Idioma: En Revista: Mol Cell Probes Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sistemas de Computación / Metiltransferasa de ADN de Sitio Específico (Adenina Especifica) / Sondas de ADN / Metilación de ADN Idioma: En Revista: Mol Cell Probes Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: China