Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis.
Stem Cell Reports
; 6(4): 525-538, 2016 Apr 12.
Article
en En
| MEDLINE
| ID: mdl-27050948
ABSTRACT
The plasticity of pluripotent stem cells provides new possibilities for studying development, degeneration, and regeneration. Protocols for the differentiation of retinal organoids from embryonic stem cells have been developed, which either recapitulate complete eyecup morphogenesis or maximize photoreceptor genesis. Here, we have developed a protocol for the efficient generation of large, 3D-stratified retinal organoids that does not require evagination of optic-vesicle-like structures, which so far limited the organoid yield. Analysis of gene expression in individual organoids, cell birthdating, and interorganoid variation indicate efficient, reproducible, and temporally regulated retinogenesis. Comparative analysis of a transgenic reporter for PAX6, a master regulator of retinogenesis, shows expression in similar cell types in mouse in vivo, and in mouse and human retinal organoids. Early or late Notch signaling inhibition forces cell differentiation, generating organoids enriched with cone or rod photoreceptors, respectively, demonstrating the power of our improved organoid system for future research in stem cell biology and regenerative medicine.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Retina
/
Células Madre Pluripotentes
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Células Madre Embrionarias Humanas
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Células Madre Embrionarias de Ratones
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Stem Cell Reports
Año:
2016
Tipo del documento:
Article
País de afiliación:
Alemania